| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 221 | Thermus thermophilus | ATCGCCATTGACCTCCACG | AGCCTTTCCGCAAGCCTT | 60.15 | 59.88 | 179 | 54 |
100.00%
|
100.00%
|
| Primer 222 | Thermus thermophilus | ATGGTTGGGGTCTTCCTGC | AAGCCCTGGAGGAAGAGGA | 59.62 | 59.52 | 188 | 54 |
100.00%
|
100.00%
|
| Primer 223 | Thermus thermophilus | GGGTCATTGAGCACGTGGA | GCCACTTGGGGTTCAGGAT | 60.00 | 59.62 | 243 | 54 |
100.00%
|
100.00%
|
| Primer 224 | Thermus thermophilus | AGTTTGCCGTCTTTGCCG | TGCTCGCCTCCTCAAACAG | 58.97 | 60.00 | 230 | 54 |
100.00%
|
100.00%
|
| Primer 225 | Thermus thermophilus | AGTTTGCCGTCTTTGCCG | TGCTCGCCTCCTCAAACA | 58.97 | 58.85 | 230 | 54 |
100.00%
|
100.00%
|
| Primer 226 | Thermus thermophilus | ATGGCACGTTTGACCGCT | CCTCCGTCTTGTTGCCCTT | 60.28 | 59.93 | 193 | 54 |
100.00%
|
100.00%
|
| Primer 227 | Thermus thermophilus | ACGTCCTGGAGCTTTACCG | TCGCCACCACGAAGGAAA | 59.41 | 59.18 | 225 | 54 |
100.00%
|
100.00%
|
| Primer 228 | Thermus thermophilus | CGAAAACGGGATCGGCATG | TGGGGTGGATGGGGATCTT | 59.65 | 59.91 | 173 | 54 |
100.00%
|
100.00%
|
| Primer 229 | Thermus thermophilus | GAAAACGGGATCGGCATGG | TGGGGTGGATGGGGATCTT | 59.27 | 59.91 | 172 | 54 |
100.00%
|
100.00%
|
| Primer 230 | Thermus thermophilus | AGGCTTTTGGACGAGGTGG | AGTCCAAGAGGTCAAAGCGG | 59.93 | 59.96 | 229 | 54 |
100.00%
|
100.00%
|